Addressing Some Critical Bottlenecks to Commercially Viable Hatchery and Nursery Techniques for Black-lip Pearl Oyster Farming in Micronesia and Population Genetics of the Black-lip Pearl Oyster (Pinctada margaritifera)
July 1, 2002
University of Hawaii at Hilo, University of Hawaii at Manoa, and Ponape Agriculture and Trade School
M. Haws, T. Lewis, S. Ellis
The overall goal of this project is to conduct applied research to address some previously identified, critical bottlenecks to black-lip pearl oyster hatchery and nursery techniques in Micronesia.
Component 1: Hatchery and Nursery Methods
- Develop simple, cost-effective, land-based early nursery techniques for black-lip pearl oysters and perform a cost-benefit analysis of these techniques compared with established ocean-based culture techniques.
- Determine natural spawning seasons for black-lip pearl oysters in Pohnpei, FSM and share data with a similar effort to be conducted at Majuro, RMI so that hatchery operators may better time their activities thus increasing the cost effectiveness of these operations. The same information will also assist in scheduling and preparing for grafting operations.
Component 2: Pearl Oyster Population Genetics
- Collect samples from hatcheries in Hawaii, FSM, and RMI as well as from natural stocks to provide genomic DNA for population genetics analysis.
- Perform amplified fragment length polymorphism (AFLP) analysis for genetic fingerprint.
- Screen microsatellite library to identify diagnostic loci for genetic fingerprint.