Project Details

Development of DNA Markers for Pacific Threadfin Aquaculture, Years 1 and 2

July 1, 2005 - July 31, 2009

LocationFundingInstitutionPrincipal Investigator(s)
Hawaii $195,884 University of Hawaii at Manoa J. Yang

Year 1

  1. To establish a genomic and skeletal muscle cDNA library of Pacific threadfin;
  2.  
  3. To identify at least 50 microsatellite loci and develop PCR protocols for optimal amplification of the markers;
  4.  
  5. To collect fin clip samples from the OI and AFRC broodstock populations and analyze polymorphic DNA markers in these samples.

Year 2

  1. To develop a microsatellite DNA-based method of parental assignment;
  2.  
  3. To collect fin clip samples from the wild populations (Oahu and Hawaiian Islands) and characterize their genetic diversities using established DNA microsatellite markers;
  4.  
  5. To develop DNA-based testing protocols for monitoring Pacific threadfin broodstock and wild populations.