Project Details

Intensified and Alternative Methods for Marine Copepod Production in Hawaii

July 1, 2007 - August 4, 2009

LocationFundingInstitutionPrincipal Investigator(s)
Hawaii $49,754 University of Hawaii Sea Grant College Program James P. Szyper, Clyde Tamaru
  1. Establish, at the Pacific Aquaculture and Coastal Resource Center (PACRC) facilities in Hilo, a project working area and one or more stock microalgal cultures (20-200 liter volumes and smaller support vessels; two or more of Chaetoceras, Isochrysis, and Tetraselmis; tended for maintenance at approx. 106 cells/mL or more as convenient) in both natural seawater and a low cost artificial seawater formulation.
  2. Obtain cultures of the marine copepods Bestiolina, Parvocalanus, and Euterpina from project work group members and other collaborators, and acclimate them to on-site conditions, including natural seawater (Criteria: survival—target =75%—through 24 and 48 hours with and without light algal feed).
  3. Document baseline (control) conditions for maintenance of the copepod cultures with microalgae in natural seawater.  The protocol will use aerated culture volumes of 1-200 liters, algal feed concentrations approx. 0.5 – 3 x 105 cells/mL, and eggs produced on normal generation cycles of approx. 2 weeks depending on species.
  4. Conduct short-term controlled experiments to assess the adaptation of the copepod cultures to artificial seawater and artificial feed particles (with quantities as in Objective 3 above), and allocate test cultures for long-term maintenance and assessment (target of 6 months at 1-10 animals per mL in 1-20 liter volumes).
  5. Technology Transfer:  Produce an article describing the context and early results of the project for the CTSA newsletter Regional Notes.
  6. Technology Transfer:  Plan the dates and content of public workshop sessions to be presented during Year 2.  Results will be presented and stakeholders will be invited to suggest further directions for project development.