Location Funding Institution Principal Investigator(s)
Hawaii $63,546

University of Hawaii at Hilo, University of Hawaii at Manoa, and Ponape Agriculture and Trade School

M. Haws, T. Lewis, S. Ellis

The overall goal of this project is to conduct applied research to address some previously identified, critical bottlenecks to black-lip pearl oyster hatchery and nursery techniques in Micronesia.

Component 1: Hatchery and Nursery Methods

  1. Develop simple, cost-effective, land-based early nursery techniques for black-lip pearl oysters and perform a cost-benefit analysis of these techniques compared with established ocean-based culture techniques.
  2. Determine natural spawning seasons for black-lip pearl oysters in Pohnpei, FSM and share data with a similar effort to be conducted at Majuro, RMI so that hatchery operators may better time their activities thus increasing the cost effectiveness of these operations. The same information will also assist in scheduling and preparing for grafting operations.

Component 2: Pearl Oyster Population Genetics

  1. Collect samples from hatcheries in Hawaii, FSM, and RMI as well as from natural stocks to provide genomic DNA for population genetics analysis.
  2. Perform amplified fragment length polymorphism (AFLP) analysis for genetic fingerprint.
  3. Screen microsatellite library to identify diagnostic loci for genetic fingerprint.
Progress Report
Location Funding Institution Principal Investigator(s)
Hawaii $10,859 University of Hawaii at Hilo M. Shintaku
Location Funding Institution Principal Investigator(s)
American Samoa $36,450 Oceanic Institute Warren G. Dominy, Ph.D.
  1. Identify, quantify and collect potential local products and byproducts for aquatic feeds development in American Samoa and dry samples for shipment to the Oceanic Institute (OI)for compositional analysis.
  2. Analyze the nutrient composition of selected samples.
  3. Compile a feed manual containing analyzed nutrition composition data for locally available ingredients and byproducts; formulated diets for Tilapia using the local ingredients; established feed processing methods and quality control tests and obtained their availability, quantity and price.
  4. Transfer of technology and dissemination of information will be achieved through a workshop and feed manual hand-outs to local producers and farmers.

 

Location Funding Institution Principal Investigator(s)
Hawaii $60,412 Oceanic Institute Dustin Moss
  1. Collect polychaetes from local near-shore habitats and screen for the incidence of shrimp viruses previously reported in Hawaii.
  2. Culture locally collected polychaetes selected based on large size and/or high reproductive output and palatability to shrimp.
  3. Culture locally collected polychaetes selected based on large size and/or high reproductive output and palatability to shrimp.
  4. Achieve settlement and metamorphosis of polychaete larvae and growth of juvenile.
  5. Select a single species for scale-up and optimization.
  6. Biochemical analysis of cultured worms to determine nutrient composition and fatty acid profiles.
  7. Disseminate the results of the research project.
Location Funding Institution Principal Investigator(s)
Hawaii $55,200 University of Hawaii, Hilo Maria Haws, Ph.D.

Overall Project Goal

The goal of this project is to develop more reliable and less expensive methods to produce triploids and tetraploids so producers do not have to rely on outside entities.

Objectives

Year 1 and 2
1. Conduct innovative research to improve production methods for triploid and tetraploid Pacific Oysters and refine and clarify published methods to suit conditions of local hatcheries.
2. Develop a pool of tetraploid specimens to be used as broodstock for Hawai`i’s hatcheries and farms. This will also support on-going efforts to selectively breed an improved oyster line(s) for Hawai`i conditions. Each farm will maintain its own tetraploid reserve which can serve as the foundation for selective breeding of lines suited for individual farms’ conditions. This will also benefit West Coast farms since Hawai`i can generate and supply broodstock more rapidly and at any time of the yea.
3. Conduct outreach to farmers, hatchery operations, students and other stakeholders. Publish clear and complete guidelines for production of triploids and tetraploids oysters allowing stakeholders access to the protocols. Although a wealth of scientific literature exists for these topics, none of it is sufficiently complete or detailed to allow others to reliably replicate the stated methods.

Location Funding Institution Principal Investigator(s)
Palau $73,524 Palau Community College, Oceanic Institute Miguel De Los Santos
  1. To develop a simple broodstock management technique for rabbitfish, S.lineatus, and produce consistent supply of eggs by spontaneous natural spawning.
  2. To develop and establish an efficient larval rearing protocol for rabbitfish, S. lineatus.
  3. WTo investigate the nursery and grow-out performance of rabbitfish, S. lineatus, fed with available commercial feeds based on growth rate, survival and feed conversion rate and demonstrate the economic viability of growing rabbitfish in tanks, ponds and cages.
  4. To transfer simple and appropriate seed production and grow-out technology to fish growers in Palau who may want to culture the rabbitfish.
Location Funding Institution Principal Investigator(s)
Commonwealth of the Northern Mariana Islands $56,256 University of Guam Hui Gong, Ph.D.
  1. Contract with local fishers for a minimum of 20 live, adult or near adult Plectropomus leopardus and 20 live adult P. laevis caught from the local wild population.
  2. Test the resulting captive stocks of these two species for the two most common classes of grouper viruses: viral encephalopathy and retinopathy also known as Viral nervous necrosis (VER/VNN), and a group of four iridoviral diseases.
  3. Maintain these stocks in quarantine and monitor them for any other diseases for a six month period.

 

Location Funding Institution Principal Investigator(s)
Hawaii $58,300 Oceanic Institute of HPU Dustin Moss

Overall Goal
The overall goal of this project is to further the prospects of commercial M. sanguinea production for use as a shrimp maturation feed.

Objectives
1. Commercial-scale growout of M. sanguinea.
2. Comparison of shrimp broodstock performance using cultured M. sanguinea and frozen, imported worms.
3. Compare worm production (growth and survival) in multiple commercially available sediments. 
4. Comparison of “multi-cohort” and “single-cohort” production systems
5. Distribute findings to industry stakeholders

Location Funding Institution Principal Investigator(s)
Hawaii $12,000 University of Florida F. Chapman
  1. Develop transportation guidelines for sturgeon embryos (seedstock), with special emphasis placed on the importance of temperature during shipment.
  2. Establish final maturation and spawning protocols for Russian sturgeon (A. gueldenstaedti), in Hawaii including short-term storage of semen for spawning synchronization.
  3. Conduct a one-day workshop on sturgeon aquaculture practices, with dual emphasis in acquisition and shipping of sturgeon livestock and in the rearing of sturgeon for meat, caviar, and broodstock.

 

Progress Report
Location Funding Institution Principal Investigator(s)
Palau $133,200 Palau Community College and Oceanic Institute of HPU Miguel A. Delos Santos and Zhi Yong Ju, Ph.D.

Overall Goal
Improve the hatchery and nursery culture technology for the mangrove crabs and to deliver a consistent production of 5,000 crablets per production unit (1.5 x 5m tank).

Objectives 
1. To improve the hatchery production by establishing a better combination of algal diets that is used to nourish the rotifers and Artemia during the larval rearing
2. To test the use of live feed enrichment products and feed additives in preventing the occurrence of mortality due to moulting death syndrome (MDS) in the larval rearing and nursery tanks.
3. To minimize the mortality due to cannibalism during the nursery rearing by evaluating other alternative types of shelters, stocking density, feeding and products containing serotonin and other chemical cues.
4. To provide support to the development of mangrove crab farming in Palau by obtaining comprehensive growth and survival data of mangrove crab juveniles that are provide to the farmers.
5. Demonstrate and disseminate an improved and reliable hatchery and nursery method for mangrove crabs to individuals who are interested in mangrove crab farming in Palau and other US Affiliated Islands in the Pacific Region.

Location Funding Institution Principal Investigator(s)
Hawaii $10,000 University of Hawaii Sea Grant Extension Service and Oceanic Institute R. Howerton and D. Ziemann
  1. Conduct a comprehensive literature review of best management practices currently in place and proposed best management practices for aquaculture systems and operations in the United States (e.g. trout, channel catfish, salmon).
  2. Review BMPs developed for other industries nationwide (beef, poultry, dairy, silviculture) and in Hawaii (sugar, pineapple) to determine how BMPs support and facilitate compliance with effluent discharge regulations.
  3. Evaluate documents generated through other Regional Aquaculture Centers concerning effluent discharge and best management practices for aquaculture.
  4. Examine international aquaculture best management practices and determine how these may apply to Hawaii aquaculture.
  5. Interact with USDA Farm Service Agency to outline BMP criteria for aquaculture farmers to follow, allowing them to be eligible for federal crop disaster assistance.
  6. The final result will be the development and distribution of one hundred copies of a practical manual outlining guidelines, recommendations and defining principles of Best Management Practices for Hawaiian Aquaculture. It is then anticipated that this manual can be used by Hawaii’s aquaculture farmers to comply with permit regulations and increase farm efficiency.

 

Location Funding Institution Principal Investigator(s)
Federated States of Micronesia $56,528 University of Hawaii at Hilo M. Haws

Component 1

  • Objective 1. Comparison of site-specific rates of snail predation for juvenile pearl oysters.
  • Objective 2. Reduction of fish-induced juvenile and adult mortality through development and testing of improved net panels.
  • Objective 3. Capacity building for a local women’s group in manufacturing of net panels.
  • Objective 4. Training and technology transfer.
  • Objective 5. Pearl farming economics study

Component 2

  • Objective 1. Demonstration and seed supply.
  • Objective 2. Training and technical transfer.

 

Progress Report
Location Funding Institution Principal Investigator(s)
Republic of the Marshall Islands $297,236 Rongelap Atoll Local Government Hon. James Matayoshi

Project Objectives:

Year 1
1. Feed Development: To collect and analyze local ingredients, and develop 5 rations for Moi trials.
2. Fish Trials: To test five (5) locally-sourced feed rations in Moi fish trials to establish the most optimal locally sourced feeds.
3. Outreach/Technology Transfer: To train local workforce on feeds and fish production.

Year 2
1. Feed Development: To develop 5 locally-sourced feed diets for Rabbitfish trials.
2. Fish Trials: To test five (5) locally-sourced feed rations in Rabbitfish trials to establish the most optimal locally sourced feeds.
3. Outreach/Technology Transfer: To train local workforce on feeds and fish production.
4. Commercialization: To conduct a marketing trial for Moi and feeds in the RMI and Hawaii to establish distribution and cost baseline.

Year 3
3. Outreach/Technology Transfer: To train local workforce on feeds and fish production.
4. Commercialization: To commercially distribute 9,800 lbs of Moi and Rabbitfish, and 14,700 lbs feeds in the RMI and Hawaii.

Location Funding Institution Principal Investigator(s)
Federated States of Micronesia $30,380 College of Micronesia R. Croft
  1. Improve the efficiency of commercial sponge farms by determining the factors responsible for variable growth rates in cultured sponges.
  2. Maintain the existing demonstration nursery.
  3. Continue gathering biological data on the growth of commercial sponges.

 

Location Funding Institution Principal Investigator(s)
Republic of the Marshall Islands $39,746 College of Micronesia R. Croft
  1. Improve the efficiency of commercial sponge farms.
  2. Maintain the existing nursery stock.
  3. Continue gathering biological data on the growth of commercial sponges.


 

Location Funding Institution Principal Investigator(s)
Federated States of Micronesia $30,000 College of Micronesia R. Croft
  1. Improve the efficiency of commercial sponge farms the investigation of sponges with large differences in growth rates, with high growth rates and low growth rates. In addition, the project will investigate surface differences between sponges.
  2. Maintain the existing nursery stock.
  3. Continue gathering biological data on the growth of commercial sponges.

 

Location Funding Institution Principal Investigator(s)
Federated States of Micronesia $40,000 College of Micronesia R. Croft
  1. Improve the efficiency of commercial sponge farms.
  2. Maintain the existing nursery stock.
  3. Continue gathering biological data on the growth of commercial sponges.

 

Location Funding Institution Principal Investigator(s)
Federated States of Micronesia $16,000 College of Micronesia R. Croft
  1. Improve the efficiency of commercial sponge farms.
  2. Maintain the existing nursery stock.

 

Location Funding Institution Principal Investigator(s)
Hawaii $100,000 University of Hawaii Sea Grant College Program Robert Howerton, Ph.D.

Year 1

  1. Characterize growth of juvenile red pacu under monoculture conditions encountered on Maui utilizing locally available commercial feeds.
  2. Compare growth and survival in monoculture and polyculture (e.g., Chinese catfish and red pacu) growout trials on Oahu.
  3. Initiate the establishment of broodstock populations for red pacu on three islands.
  4. Obtain and collate technical information available on pacu including those written in foreign languages.
  5. Locate sources of the black pacu that meet import requirements for the state of Hawaii.
  6. Conduct technology transfer to appropriate end users.

Year 2

  1. Conduct pilot-scale monoculture growout enterprises in collaboration with private sector farms.
  2. Characterize growth and survival of juvenile red pacu in closed recirculating systems.
  3. Test market pacu as a food fish species on both Oahu and Maui and conduct survey of ornamental market demand.
  4. Technology transfer to appropriate end users.

 

Location Funding Institution Principal Investigator(s)
Hawaii $71,182 University of Hawaii at Hilo Maria Haws, Ph.D.
  1. Assess the effects of seasonality on reproduction and condition index (CI) for Crassostrea gigas in Hawai`ian fishponds and other sites to determine ideal harvest and stocking schedules and maximum stocking densities.
  2. Improve triploid C. gigas performance by testing different triploid crosses. The triploid crosses will be produced by using lines developed by the Molluscan Broodstock Program (MBP) and the Midori C. gigas strain as the diploid parents to be crossed with tetraploid oysters. Crosses will be tested in Hawai`ian fishponds and other sites to determine whether these triploids differ in performance from previous triploids crosses.
  3. Develop a high-value half-shell oyster product for Hawai`i by conducting grow-out trials of a newly developed indigenous oyster species (Dendrostea sandvichensis) and Kumamoto oysters (C. sikamea).
  4. Test culture methods for Manila clams to improve survival and growth.
  5. Test a new broodstock conditioning system for Tellina palatum (Hawai`ian clams) to improve on previous spawning trials.
  6. Conduct outreach to transfer the technology developed in Objectives 1-5.
Location Funding Institution Principal Investigator(s)
Palau $297,400 Oceanic Institute Chatham K. Callan, Ph.D

Project Objectives:

Year 1
1. Establish and maintain broodstock populations of coral grouper and monitor egg production at BMR and PCC facilities.
2. Establish cultures of Parvocalanus copepods at PCC hatchery for use in testing on grouper larvae.
3. Optimize and refine culture methods for suitable species of copepods for testing on grouper.

Year 2
4. Continue evaluation of broodstock maturation over multiple spawning cycles.
5. Provide training workshop on intensive copepod production and hatchery methods.
6. Establish optimal feeding protocol for coral grouper larvae.

Year 3
7. Provide onsite copepod-based, grouper culture training at Palau hatchery.
8. Create a technical manual for the use of copepods in grouper culture.
9. Conduct final workshop to review project accomplishments and discuss future opportunities.

Location Funding Institution Principal Investigator(s)
Republic of the Marshall Islands $145,000 Rongelap Atoll Local Government Ryan Murashige

Overall Goal
The overall goal of the 2-year project is to establish economically viable management for marine finfish aquaculture (Moi) and local aquatic feed production in the Marshall Islands and to transfer that knowledge through training programs to the RMI current and future project staff.

Objectives 
Year 1
1. Feed Development: To conduct feeding trials (control and dry) in order to establish the most optimal and cost-efficient feeds for Moi
2. Outreach/Technology Transfer: To train the local workforce on feeds and fish production.
Year 2
3. Marine Aquaculture Technology Development: To conduct two (2) fish density trials to establish the most optimal and economical cage culture grow-out requirements for Moi.
4. Outreach/Technology Transfer: To train the local workforce on feeds and fish production.

Location Funding Institution Principal Investigator(s)
Palau $128,810 Palau Community College Miguel Delos Santos

Project Objectives:

Year 1:
1. To collect sexually matured milkfish and establish at least two broodstock facilities that will be monitored for natural spawning in captivity.
2. To document the egg production performance of captive milkfish broodstock in Palau condition.
3. To adopt and verify a current commercial hatchery methods for mikfish fry production in Palau.

Year 2:
4. To optimize milkfish fry production by incorporating other finfish hatchery technique.
5. To conduct a hands-on training to existing local finfish hatchery operators and produce a simple and practical extension manual and for milkfish fry production in Palau.

Location Funding Institution Principal Investigator(s)
Guam $52,964 University of Guam D. Crisostomo
  1. Establish a raceway-based hatchery at the Guam Aquaculture Development and Training Center with the broodstock matured during the first year of the project.
  2. Determine reproductive rates for each of the five tilapia strains under investigation.
  3. Determine the management requirements for broodstock in a raceway/hapa system.
  4. Combine the information on growth and reproduction of each of the five strains in an economic analysis to decide which two strains will be kept as the basis for a self-sufficient tilapia industry on Guam.
  5. Conduct a one-week hands-on training for aquaculture personnel from Saipan and Rota on the operation and management of a tilapia hatchery, and conduct site analyses of their respective facilities to assist them in establishing their own tilapia hatcheries.

 

Progress Report
Location Funding Institution Principal Investigator(s)
Guam $50,200 University of Guam D. Crisostomo

To establish a functional tilapia hatchery on Guam for a self-sufficient industry using the best two strains of improved tilapia identified by this project.

  1. Identify, obtain and import into Guam male seedstock of five strains of tilapia.
  2. Evaluate the growth rate, survival, food conversion, and dress out characteristics of these five strains under local conditions in both ponds and above ground tank systems.
  3. Obtain, import and raise to sexual maturity broodstock of the five strains for future determination of reproductive rates and estimation of the costs of fry production at the GADTC facility.

 

Progress Report
Location Funding Institution Principal Investigator(s)
Hawaii $110,000 University of Hawaii Sea Grant Extension Service and University of Hawaii at Manoa C. Tamaru, R. Bailey, C. Brown, B. Cole, and H. Ako
  1. Amplify and distribute high health broodstock to commercial culturists in Hawaii.
  2. Confirm stocking densities for at least two ornamental species that result in optimal growth and survival.
  3. Demonstrate out of season maturation and spawning of at least one freshwater ornamental fish species (i.e. rainbow or redtail sharks).
  4. Demonstrate optimal stocking densities of one ornamental fish species in a commercially available recirculating system and determine economic feasibility.
  5. Complete a paper study of aquatic ornamental plants that hold potential for culture in Hawaii.
  6. Conduct technical workshops, provide on-site extension, verbal consultations and literature to develop ornamental fish culture in Hawaii.

 

Location Funding Institution Principal Investigator(s)
Hawaii $50,000 University of Hawaii Sea Grant Extension Service and University of Hawaii at Manoa C. Tamaru, B. Cole, R. Bailey, and C. Brown
  1. Expand production and distribution of tropical fish species and add new farms to production in Hawaii.
  2. Operate an incubator hatchery for the production of 2-week-old larvae of selected egg-layers for distribution to prospective farmers.
  3. Expand technical assistance to more demonstration farm sites and include small-scale commercial breeders to increase production and diversification of species.

 

Location Funding Institution Principal Investigator(s)
Hawaii $100,000 University of Hawaii Sea Grant Extension Service and University of Hawaii at Manoa C. Tamaru, R. Bailey, B. Cole, and C. Brown
  1. Expand the current number of farms/individuals commercially producing freshwater ornamentals and diversify tropical fish culture by incorporating an additional five species to the inventory already being supported by CTSA.
  2. Collaborate with the private sector and the state aquatic veterinarian with the importation of additional species by providing facilities and maintenance during quarantine period where diagnostics to screen incoming fish for pathogens will also be provide.
  3. Introduce production/marketing infrastructure scenarios to tropical fish growers in Hawaii.
  4. Conduct extension activities in the form of technical workshops (n=3), site visitation, verbal consultations and providing literature in support of development of additional farms geared towards the culture of freshwater tropical fish in Hawaii.

 

Location Funding Institution Principal Investigator(s)
Hawaii $90,000

University of Hawaii Sea Grant Extension Service and University of Guam

J. Szyper and D. Crisostomo

Year 1 Hawaii Component:

  1. Establish a tilapia quarantine and project working area at the University of Hawaii at Hilo aquaculture program facility. All existing tilapias will be eliminated from the site before the project begins.
  2. Obtain permits and import:
  3. a.fingerling blue tilapia, Oreochromis aureus (a state-permitted species on list C: permitted for commerce) from a suitable commercial farm source that will guarantee disease-free stock. b.fingerling Nile tilapia, Oreochromis niloticus (recently approved for list A: restricted, research) from a similar commercial source.
  4. Quarantine the imported stocks (both species, if permits are granted) pending examination, and obtain certification of disease-free status by the state fish health management program (which has agreed to assist with this specific project).
  5. Grow and mature breeding stocks from the imported population(s), both species, if permits are granted.
  6. Produce offspring from the new breeder stocks of O. aureus, and use them to institute a breeding management program to preserve their genetic quality into future generations.
  7. Obtain at least three existing commercial tilapia stocks from within the state (one each from Oahu, Hawaii, and Maui islands), and perform the first growth comparison trial between in-state and imported stocks (both species, if permits are granted). Also, send fingerling O. aureus to Oahu for comparison trials there.
  8. Characterize the imported (both species) and comparison stocks for genetic identification by microsatellite DNA markers. This will be done for each stock at the beginning of growth trials and for the imported stocks near the end of the project.
  9. Communicate the first year’s results (broodstock management protocols, growth comparisons, draft of BMP manual) in at least two public workshops and in widely accessible written materials (newsletter articles, web postings) apart from project reports.


Year 1 Guam Component (formerly Year 3 of Guam project):

  1. Produce offspring from breeder stocks developed during Year 2 work (two best types for reproductive capacity) and institute a breeding management program to preserve their genetic quality into future generations. It may be necessary to import additional broodstock of these two types. The breeder management program will be analogous to that for Hawaii Year 1 Objective 5 above, with modification for Guam site conditions.
  2. Send samples of fish from each of the lines established for breeder management to Hawaii for genetic testing.
  3. Distribute modest numbers of fingerlings to the community for examination and testing on farm sites, subject to protocols and local shared agreements to avoid interference with markets.
  4. Evaluate and improve production protocols established in Year 2.

 

Progress Report
Location Funding Institution Principal Investigator(s)
Hawaii $52,730

University of Hawaii Sea Grant College Program

J. Szyper

  1. To complete the first growth comparison trials, begun in Year 1 of the Hawaii component with extension to approximately 400 g final fish size. The original objective was stated: “To obtain at least three existing commercial tilapia stocks from within the state (one each from Oahu, Hawaii, and Maui islands), and to perform the first growth comparison trial between in-state and imported stocks (both species if permits are granted). Also, to send fingerling O. aureus to Oahu for comparison trials there. Deliverable: Description of trial, photos of fish, written results and interpretation. Accomplishment stated and photos reproduced for project reports, workshops, newsletters, etc.”
  2. To obtain at least two additional in-state stocks and to perform further experimental comparisons of growth potential.
  3. To distribute fingerlings of imported O. aureus stocks (O. niloticus is not permitted for distribution from the farm) to interested businesses for on-site testing, under terms to be established with community input, avoiding interference with markets. This item may begin late in Year 1 if sufficient fish are available after allocation to growth trials.
  4. To integrate the work on importation, quarantine, growth comparisons and stock maintenance protocols into a manual on best management practices, and to offer public workshops covering this information during the last half of the project.
Progress Report
Location Funding Institution Principal Investigator(s)
Hawaii $49,180 University of Hawaii at Hilo, Hawaii Division of Aquatic Resources, University of Hawaii at Manoa, and Okamura Fish Farms K. Hopkins, L. Hallacher, T. Iwai, G. Grau, and W. Okamura
  1. Review the international, national, and Hawaii technical and regulatory status of tilapia stocks and strains as related to improvement of the tilapia aquaculture industry in Hawaii.
  2. Morphometrically and genetically assess the status of farmed stocks from 12 sites and wild tilapia stocks from 12 sites on six Hawaiian islands.
  3. Determine growth characteristics of existing farmed tilapias, and compare the data with available scientific literature.
  4. Recommend measures to improve existing stocks with the available stocks, or propose importations of new tilapia strains or species based upon review of the collected information.

 

Location Funding Institution Principal Investigator(s)
Hawaii $46,090 Oceanic Institute of HPU Chatham K. Callan, PhD and Dustin Moss

Overall Goal
The overall goal of this project is to improve and expand fish culture in the region by addressing a significant bottleneck in the production of copepod nauplii, a critical larval food for many marine species. Specifically, we aim to improve the efficiency of copepod nauplii production using selective breeding to develop line(s) of copepods that are highly fecund at high culture densities.

Objectives
1: Establish experimental copepod populations maintained at increased culture densities (3 and 6 adults/mL).
2: Use mass selection to select for nauplii production at high-culture density for at least 6 generations (120 days).
3: Compare nauplii production between selected lines (3 and 6 adults/mL) and control line (1.5 adults/mL) in a commercial scale, high-density production system.
4: Conduct final workshop to review project accomplishments and discuss future opportunities.

Location Funding Institution Principal Investigator(s)
Palau $43,093 Oceanic Institute Zhi Yong Ju, Ph.D., Miguel Delos Santos

Overall Project Goal

The project’s overall goals are to support the growing interest in mangrove crab farming in Palau through the establishment of nursery and grow-out culture, and to advance aquaculture techniques that improve survival rate and feeds efficiency.

Objectives

1. Test the effects of different types of shelters on survival rates to market size for mangrove crab juveniles in grow-out facilities.
2. Identify and secure local feed ingredients for nutritional analysis and prepare nutritive feed supplement pellets and a functional feed supplement to be applied to imported shrimp feeds.
3. Improve culture performance of juvenile mangrove crabs by combination of imported shrimp feed with nutrient and functional feed supplements.

Location Funding Institution Principal Investigator(s)
Federated States of Micronesia $97,100 College of Micronesia M. Ito
  1. Improve roundness rate by grafting techniques.
  2. Reduce flaws by grafting techniques and husbandry methods.
  3. Improve host’s survivorship by husbandry methods.
  4. Transfer pearl aquaculture technology immediately to Micronesia and other regions.

 

Progress Report
Location Funding Institution Principal Investigator(s)
Palau $92,268 Palau Community College Miguel Delos Santos

Overall Project Goal

The project’s overall goal is to improve upon existing rabbitfish culture technologies in Palau with the main objectives of improving hatchery survival and increasing subsequent fingerling production of these highly important species in the USAPI region.

Objectives

Year 1
1. Refine broodstock holding and egg handling techniques for Siganus lineatus to facilitate more reliable egg supply and hatchery production.
2. Improve larval rearing protocols for Siganus lineatus to increase larval survival and production of juveniles.

Year 2
3. Scale up production of Siganus lineatus and to provide sufficient number of juveniles for subsequent grow-out at local farms.
4. Provide onsite training to local stakeholders on rabbitfish broodstock handling and hatchery production methods

Location Funding Institution Principal Investigator(s)
Hawaii $18,635 University of Hawaii at Hilo K. Hopkins
  1. Training. Improve the level of technical expertise of at least six Hawaiian fish farmers so that they can nurse, handle, stage, and if possible, spawn A. gueldenstaedti.
  2. Establish Sturgeon Stocks. Establish at least three (six preferred) populations of A. gueldenstaedti and one other Russian species at farms throughout Hawaii.
  3. Reducing Deformity. Determine if increasing calcium concentrations in incubation water will reduce the incidence of deformity in yolk sac larvae and fingerlings.
  4. Use of Commercial Fry Feeds. Determine if readily available commercial feeds can be used effectively to grow the three species of Russian sturgeons from fry to fingerling (100g) stage.
  5. Hatchery Manual. Prepare a preliminary hatchery manual for Russian sturgeons in Hawaii.

 

Progress Report
Location Funding Institution Principal Investigator(s)
Hawaii $11,335 University of Hawaii R. Howerton
  1. Develop larval rearing techniques for one and possibly two species of sturgeon, A. gueldenstaedti and Huso huso.
  2. Determine optimal feeding protocols for sturgeon larvae and fry and improve survival rates during transition from live feeds to artificially prepared diets.
  3. Determine optimal temperature requirements for sturgeon larvae and fry culture.

 

Progress Report
Location Funding Institution Principal Investigator(s)
Hawaii $83,306 University of Hawaii, Hilo (PACRC) Maria Haws, Ph.D.

Project Objectives:

Year 1 and 2
1. Design, develop and test depuration units that are suitable to local conditions, cost-effective and in compliance with the unique Hawaii DOH regulations. Year 1.
2. Determine if depuration affects oyster quality and determine if salting enhances the lepto-organic properties of oysters grown in Hawaiian fishponds. Year 1.
3. Quantify Vibrio vulnificus and V. parahaemolytus in pond water and in oyster tissues before and after depuration. Year 1-2.
4. Design, build and test a nursery system that is suitable for conditions found in Hawaiian fishponds. Year 1-2.
5. Train farmers in two critical industry topics: 1) depuration and food safety; 2) and setting and nursery methods. Year 1-2.

Location Funding Institution Principal Investigator(s)
Hawaii $38,802 Oceanic Institute Dustin Moss, Ph.D.

Overall Project Goal

The goal of the current proposal is to test the feasibility of an IMTA approach using sea cucumbers to improve nutrient use efficiency and to create an additional revenue stream for shrimp farms.

Objectives

Year 1 and 2
1. Identify, determine availability, collect, and screen for shrimp pathogens for a minimum of two commercially-valuable species of sea cucumber that naturally occur in Hawaiian near-shore waters.
2. Conduct replicated sea cucumber culture experiments to quantify growth, survival, nutrient flow, and sludge (produced from shrimp RAS) processing capability.
3. Conduct two IMTA demonstration trials: 1- sea cucumbers fed sludge from a shrimp RAS while reared in a separate culture system and 2- polyculture of shrimp and sea cucumbers in a traditional open pond.
4. Disseminate research results to interested stakeholders in the USAPI and to a broader aquaculture community.

Location Funding Institution Principal Investigator(s)
Hawaii $70,000 Oceanic Institute C. Laidley

Year 1:

  1. Complete industry survey of algal production requirements.
  2.  
  3. Generate a current review of available algal bioreactor systems examining costs of setup and operation for publication in CTSA notes.
  4.  
  5. Commission pilot-scale algae production system(s) to evaluate actual cost and ease of setup.
  6.  
  7. Conduct on-site operation and testing of algal photo-bioreactors with several species of marine and freshwater microalgae.
  8.  
  9. Provide education and training to the aquaculture industry through workshops, fact sheets, and continuing education programs at the new Oceanic Institute Learning Center.

Year 2 (contingent on results of Year 1):

  1. Complete comparative cost analysis of plate and cylinder-based photo-bioreactors for comparison to classical tank-based batch culture production systems.
  2.  
  3. Review the performance of algal photo-bioreactors in commercial settings on multiple islands in Hawaii and the Pacific.
  4.  
  5. Conduct workshops at multiple sites in Hawaii and the Pacific.
  6.  
  7. Establish permanent demonstration systems at the Oceanic Institute Learning Center for continued long-term use in training of industry and students.

 

Progress Report
Location Funding Institution Principal Investigator(s)
Hawaii $21,300 University of Hawaii Sea Grant Extension Service and University of Guam P. Olin, B. Miller, R. Bailey, D. Crisostomo, and B. Smith

Project I: Introduction of the warm-water abalone species Haliotis diversicolor supertexta and H. fulgens for culture assessment in Hawaii

  1.   Document the review process for import permit applications, and prepare scientific guidelines for preparation and submission of permit applications. These guidelines will be published as an extension fact sheet.
  2.  
  3.   Conduct a literature search, and prepare a permit application to import Haliotis diversicolor supertexta and H. fulgens. Identify appropriate quarantine facilities and sources of abalone seed. The importation process, quarantine requirements, and sources of abalone seed will be published as an extension fact sheet.
  4.  
  5.   Import abalone species, and conduct grow-out trials at two commercial farms in Hawaii. Results of the grow-out trials will be disseminated in a brochure detailing results and recommendations for abalone culture in Hawaii. If sufficient interest exists, a workshop will be presented to the aquaculture community

.

Project II: Introduction of the Clam Meretrix lusoria for Biological Culture Assessment in Guam

  1. Clarify and document the procedure necessary for the importation of new species for aquaculture in Guam.
  2.  
  3. Import to Guam the clam Meretrix lusoria for biological culture assessment.

 

Location Funding Institution Principal Investigator(s)
Hawaii $50,740 University of Hawaii Sea Grant Extension Service, University of Hawaii at Manoa, and University of Florida B. Miller, P. Olin, R. Bailey, C. Brown, and F. Chapman
  1. Import and evaluate the culture potential of the green-lip abalone, Haliotis laevigata.
  2. Obtain a market assessment of the tropical ornamental fish and aquatic plant industry.
  3. Import and evaluate the culture potential of selected species of freshwater ornamental fish including optimum grow-out methods and economic evaluation.
  4. Develop protocols for larval rearing and grow-out of the silver arowana, Osteoglossum bicirrohosum.
  5. Develop production methods for Tubifex worms and evaluate

 

Location Funding Institution Principal Investigator(s)
Hawaii $151,800 Oceanic Institute of HPU and Palau Community College Chatham K. Callan, Ph.D. and Miguel Delos Santos

Overall Goal
The project’s overall goals are to further refine and optimize the production technology for Coral Grouper and to help determine the economic feasibility for producing this species.
 
Objectives
Year 1
1. Maintain and expand broodstock populations of coral grouper and monitor egg production at OI.
2. Evaluate the grow-out of coral grouper fingerlings on shore.
3. Refine hatchery technology to further improve survival and efficiency of coral grouper fingerling production.

Year 2
4. Continue evaluation of broodstock maturation, including F1 stocks, over multiple spawning cycles.
5. Continue to evaluate the grow-out of coral grouper fingerlings on shore and complete cost of production analysis. 
6. Conduct final workshop to review project accomplishments and discuss future opportunities.

Location Funding Institution Principal Investigator(s)
Hawaii $122,200 University of Hawaii at Manoa, University of Hawaii Sea Grant Extension Service, and University of Florida C. Brown, R. Bailey, B. Cole, J. Brock, and F. Chapman
  1. Retain an ornamental fish production and breeding specialist to provide technical support to farmers producing ornamental fish. Technology transfer will include breeding protocols, growout, disease and predator management, marketing, packaging and shipping.
  2. Establish the consistent production of five species of high health ornamental fish at commercial farm locations in Hawaii.
  3. Establish high health stocks of additional species of ornamental fish for distribution to participating farmers.
  4. Continue the market assessment and conduct a business feasibility analysis in conjunction with the Pacific Business Center to identify production costs, markets and financial feasibility of ornamental production in Hawaii.
  5. Reproduce Amazon Basin fish using reverse osmosis technology.

 

Location Funding Institution Principal Investigator(s)
Hawaii $70,000 University of Hawaii at Manoa and University of Hawaii Sea Grant Extension Service C. Tamaru, B. Miller, B. Cole, R. Bailey, and C. Brown
  1. Retain an ornamental fish production and breeding specialist.
  2. Provide seedstock and technical support to farmers.
  3. Produce a manual detailing culture methods.
  4. Develop breeding and production protocols for selected ornamental fishes.

 

Location Funding Institution Principal Investigator(s)
Federated States of Micronesia $69,836 College of Micronesia Land Grant Program Masahiro Ito

1. Search and collect sandfish (H. Scabra) broodstock in the Pohnpei lagoon.

2. Conduct broodstock conditioning, spawning induction, and larval rearing.

3. Develop a method for sandfish juvenile production in Pohnpei.

4. Train Micronesians in sea cucumber hatchery operation.

Location Funding Institution Principal Investigator(s)
Palau $31,690 Palau Community College TBD
  1. To collect locally available mangrove crab (Scylla serrata) broodstock and document their spawning in captivity.
  2. To verify the production performance of the available hatchery and nursery techniques in producing mangrove crab seedstock in terms of growth rate and survival rate in Palau conditions.
  3. To determine the economic feasibility of producing mangrove crab seedstock under Palau conditions.
  4. To demonstrate and extend a practical mangrove crab hatchery technology to the stakeholders.

 

Location Funding Institution Principal Investigator(s)
American Samoa $25,000 Oceanic Institute and Anuenue Fisheries Research Center A. Ostrowski, B. Keala, and M. Fujimoto
  1. Produce seedstock of milkfish (Chanos chanos) from a hatchery.
  2. Conduct growout and baitfish trials with hatchery seedstock.
  3. Conduct a milkfish hatchery feasibility study.

 

Location Funding Institution Principal Investigator(s)
Federated States of Micronesia $10,500 College of Micronesia and Pohnpei Marine Resources Division R. Croft and F. Curren
  1. Establish a small-scale sponge demonstration farm; provide preliminary data on growth of bath sponges following cutting and planting in the FSM.
  2. Prepare a long-range plan for developing the sponge aquaculture industry in the FSM.

 

Location Funding Institution Principal Investigator(s)
Federated States of Micronesia $10,900 College of Micronesia R. Croft
Location Funding Institution Principal Investigator(s)
Federated States of Micronesia $30,450 College of Micronesia and Pohnpei Marine Resources Division R. Croft and F. Curren
  1. Continue gathering biological data on the growth of commercial sponges.
  2. Maintain and expand the existing stock of cultured sponges.
  3. Start training local sponge farmers and assist them with starting their private farms.

 

Location Funding Institution Principal Investigator(s)
Federated States of Micronesia $38,550 College of Micronesia R. Croft
  1. Continue gathering biological data on the growth of commercial sponges.
  2.  
  3. Maintain an existing demonstration/nursery sponge farm.
  4.  
  5. Continue training local sponge farmers, and assist them with starting their private farms.

 

Location Funding Institution Principal Investigator(s)
American Samoa $24,615 College of Micronesia R. Croft
  1. Provide extension assistance to established farms.
  2. Train local sponge farmers and assist them with starting their private farms.
  3. Present a summary of findings/activities at national and regional meetings.

 

Location Funding Institution Principal Investigator(s)
Hawaii $75,000 University of Hawaii Sea Grant Extension Service C. Tamaru
  • Objective 1. Invite industry expert(s) to partcipate in a conference on marketing trends in the freshwater ornamental fish industry.
  • Objective 2. Investigate use of carotenoids for color enhancement.
  • Objective 3. Validate techniques for artificial insemination of live bearers.
  • Objective 4. Determine minimum effective dosage of Ovaprim for induction of spawning.
  • Objective 5. Validate factors affecting the sex ratio in swordtails.
  • Objective 6. Conduct technology transfer activities.

 

Progress Report
Location Funding Institution Principal Investigator(s)
Hawaii $70,000 University of Hawaii Sea Grant Extension Service C. Tamaru
  1. Produce a position paper summarizing the current status of Hawaii’s freshwater ornamental fish industry.
  2. Field test the artificial insemination technique to increase efficiency in the commercial production of lyretail swordtails.
  3. Demonstrate the cost effectiveness of specialization in the production of freshwater ornamental fishes.
  4. Provide technical assistance in the form of workshops, verbal communication, written material and site visits.

 

Progress Report
Location Funding Institution Principal Investigator(s)
Hawaii $60,000 University of Hawaii at Hilo, University of California, Santa Cruz, Hawaii Aquaculture Development Program, and Brookwood Biomedical Lab M. Shintaku, L. Groff, J. Brock, and R. Lallone
  1. Demonstrate under controlled laboratory conditions the transmission and infectivity of the GGS agent.
  2. Describe in detail by light microscopy the external and internal characteristics of Gracilaria tikvahiae affected by GGS.
  3. Characterize the bacterial communities associated with Gracilaria tikvahiae unaffected and affected by GGS.
  4. Develop a probe for identification of biochemical markers of GGS or the GGS agent.