Development of DNA Markers for Pacific Threadfin Aquaculture, Years 1 and 2
Location | Funding | Institution | Principal Investigator(s) |
---|---|---|---|
Hawaii | $195,884 | University of Hawaii at Manoa | J. Yang |
Year 1
- To establish a genomic and skeletal muscle cDNA library of Pacific threadfin;
- To identify at least 50 microsatellite loci and develop PCR protocols for optimal amplification of the markers;
- To collect fin clip samples from the OI and AFRC broodstock populations and analyze polymorphic DNA markers in these samples.
Year 2
- To develop a microsatellite DNA-based method of parental assignment;
- To collect fin clip samples from the wild populations (Oahu and Hawaiian Islands) and characterize their genetic diversities using established DNA microsatellite markers;
- To develop DNA-based testing protocols for monitoring Pacific threadfin broodstock and wild populations.
Regions
CTSA
Contact information:
University of Hawaii, Agricultural Science 216
1955 East West Road
Honolulu, HI 96822
Telephone: (808) 956-3974