Development of DNA Markers for Pacific Threadfin Aquaculture, Years 1 and 2
|Hawaii||$195,884||University of Hawaii at Manoa||J. Yang|
- To establish a genomic and skeletal muscle cDNA library of Pacific threadfin;
- To identify at least 50 microsatellite loci and develop PCR protocols for optimal amplification of the markers;
- To collect fin clip samples from the OI and AFRC broodstock populations and analyze polymorphic DNA markers in these samples.
- To develop a microsatellite DNA-based method of parental assignment;
- To collect fin clip samples from the wild populations (Oahu and Hawaiian Islands) and characterize their genetic diversities using established DNA microsatellite markers;
- To develop DNA-based testing protocols for monitoring Pacific threadfin broodstock and wild populations.