Location Funding Institution Principal Investigator(s)
Hawaii $195,884 University of Hawaii at Manoa J. Yang

Year 1

  1. To establish a genomic and skeletal muscle cDNA library of Pacific threadfin;
  2.  
  3. To identify at least 50 microsatellite loci and develop PCR protocols for optimal amplification of the markers;
  4.  
  5. To collect fin clip samples from the OI and AFRC broodstock populations and analyze polymorphic DNA markers in these samples.

Year 2

  1. To develop a microsatellite DNA-based method of parental assignment;
  2.  
  3. To collect fin clip samples from the wild populations (Oahu and Hawaiian Islands) and characterize their genetic diversities using established DNA microsatellite markers;
  4.  
  5. To develop DNA-based testing protocols for monitoring Pacific threadfin broodstock and wild populations.

 

CTSA

Contact information:

University of Hawaii, Agricultural Science 216
1955 East West Road
Honolulu, HI 96822

Telephone: (808) 956-3974

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