Location	Funding	Institution	Principal Investigator(s)
Hawaii	$195,884	University of Hawaii at Manoa	J. Yang

Year 1

1. To establish a genomic and skeletal muscle cDNA library of Pacific threadfin;
 
2. To identify at least 50 microsatellite loci and develop PCR protocols for optimal amplification of the markers;
 
3. To collect fin clip samples from the OI and AFRC broodstock populations and analyze polymorphic DNA markers in these samples.

Year 2

1. To develop a microsatellite DNA-based method of parental assignment;
 
2. To collect fin clip samples from the wild populations (Oahu and Hawaiian Islands) and characterize their genetic diversities using established DNA microsatellite markers;
 
3. To develop DNA-based testing protocols for monitoring Pacific threadfin broodstock and wild populations.

 

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