Location	Funding	Institution	Principal Investigator(s)
Hawaii	$74,439	University of Hawai’i at Mānoa and HDOA	Jenee Odani, Ph.D., Lei Yamasaki, Ph.D., RuthEllen Klinger-Bowen

Overall Goal
To determine which diseases affect fish producers in Hawaii, determine if non-lethal methods of testing can be used for import and export purposes and investigate whether pathogens enter the state through seafood.

Objectives
1. Determine what diseases are of concern to fish and shellfish producers, state and federal government and importing countries.
2. Set up polymerase chain reaction (PCR) tests for the specific pathogens at the UHM. Compare the test results of lethal and non-lethal methods. This will be based on the list of disease priorities generated in Objective 1, which may include, but is not limited to: Tilapia Lake Virus (TiLV), Koi Herpesvirus (KHV), Spring Viremia of Carp (SVC), Viral Nervous Necrosis (VNN), Grouper Iridovirus (GIV), and Francisella noatunensis subsp. orientalis (FNO) in fish and Ostreid Herpesvirus-1 (OsHV-1) in oysters.
3. Conduct surveillance of imported fish and oysters for pathogen presence. This may include, but is not limited to TiLV, KHV, SVC, VNN, GIV, FNO, and Vibrio anguillarum (VA) in fish and OsHV-1 in oysters.