Location	Funding	Institution	Principal Investigator(s)
Hawaii	$49,000	University of Hawaii at Manoa	Michael Wong, RuthEllen Klinger-Bowen, Lei Yamasaki

Overall Project Goal
The goal of the project is to determine the prevalence of Fno on islands other than Oahu, what species are infected and established in natural bodies of water in Hawaii.

Objectives
1. Collect up to 400 specimens of tilapia from feral populations on the islands of Hawaii, Kauai, Maui, Molokai and Oahu.
2. Necropsy the specimens, record any gross findings associated with Fno infection, culture spleens for microbiological analysis, and collect sample tissues of spleen for histopathology and PCR.
3. Screen sample tissues of spleen for Fno infection using conventional PCR methods
4. Perform fin biopsies and submit samples to the laboratory at Georgia College and State University for tilapia species identification via molecular methods.
5. Prepare articles to the scientific community and in lay terms to convey the project information to the aquaculture industry and regulatory agencies.