Improving Cost-effectiveness of Producing Local Aquatic Feed from Papaya Fruit Wastes via Innovative Bioprocessing, Years 1 and 2 | Center for Tropical and Subtropical Aquaculture

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Location	Funding	Institution	Principal Investigator(s)
Hawaii	$75,000	University of Hawai’i at Mānoa	Wei Wen Su, Ph.D. and Armando García-Ortega Ph.D.

Overall Goal
The goal of this project is to conduct a small-scale feasibility study of enriching papaya fruit wastes with protein-rich yeast and autolysate derived from the yeast, as protein/amino acid supplements in local aquaculture feed, using a low-cost semi-solid state fermentation method.

Objectives
1. Process papaya culls into nutrient substrates suited for semi-SSF of Yarrowia yeast, using simple affordable mechanized processing that is amenable to common commercial operations, and adoptable by local growers and producers.
2. Optimize and scale up semi-SSF of Y. lipolytica using waste papaya fruit pulp and seed oil as carbon sources
3. Assess the feasibility of using intact yeast biomass and autolyzed yeast extract as feed ingredients in aquaculture feeds by way of its nutritional composition analysis and an 8-week growth trial in tilapia (Oreochromis niloticus).