What’s in the gut? A Metabarcoding Approach to Examining Diet in First Feeding Larvae | Center for Tropical and Subtropical Aquaculture

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Location	Funding	Institution	Principal Investigator(s)
Hawaii	$67,519	Hawaii Institute of Marine Biology	Brian Bowen and Robert Toonen

Overall Goal
This project will harness advanced genetic technology (next-generation sequencing) for the benefit of aquaculture, by generating informed breeding-rearing protocols for the marine aquaculture industry.

Objectives
1. Obtain larvae of target species from the wild for robust diet analyses. NOAA partner Robert Humphreys has extensive frozen archives of larvae from plankton tows.
2. Produce and identify DNA barcodes from stomach contents of marine fish larvae, using high-throughput DNA sequencing technology.
3. Produce a database of potential prey items.